cell immunofluorescence staining cells  (Proteintech)

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Genomic alteration profiles of NCBP1, NCBP2, NCBP3, NCBP2-AS1, NCBP2AS2 and NCBP2L in TCGA pan-cancer cohort. (B–C) Kaplan–Meier survival analysis showed OS (B) and DFS (C) in all pan-cancer patients divided by NCBP2 altered or unaltered groups. (D) The genomic alteration of NCBP2 in TCGA pan-cancer atlas, including mutation, amplification, deep deletion and multiple alterations.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Mutagenesis, Amplification

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The relative mRNA expression level of NCBP2 between tumor and normal tissues in pan-cancer from TCGA cohort. (B) The expression of NCBP2 in several cancer cell lines, including U-251, A-431, U2-OS, PC3 and MCF7 were detected by immunofluorescent staining in Human Protein Atlas. (C–F) The protein expression of NCBP2 in LUSC (C), KIRP (D), PAAD (E) and KIRC (F) and paired normal tissues were detected by immunohistochemical stain. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The correlation between NCBP2 and patients’ OS were shown by forest plot. (B–G) Kaplan–Meier survival analysis for patients with specific cancers (UCEC, PAAD, LIHC, KIRC, KICH and KIRP) base on the expression of NCBP2. (H–M) Multivariate cox regression analyses evaluated the prognostic independence of NCBP2 and clinicopathological features regarding OS of UCEC, PAAD, LIHC, KIRC, KICH and KIRP in TCGA datasets.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: Differential expressed genes (DEGs) were analyzed between the top and bottom 30% NCBP2 expression subgroup for each cancer in TCGA, following hallmarks gene set enrichment analysis (GSEA) of those DEGs to investigate the NCBP2-associated cancer processes. The size of the circle represents the false discovery rate (FDR) adjusted P value of each cancer enrichment item, and the color represents the normalized enrichment score (NES) of each enrichment item.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Heatmap showing the correlation of NCBP2 expression and stromal score, immune score, and ESTIMATE score in pan-cancer. Positive correlation in red and negative correlation in blue. (B) Heatmap showing the correlation of NCBP2 and infiltration levels of immune cells including CD4+ T cells, CAF, progenitor, Endo, Eos, HSC, Tfh, gdT, NKT, regulatory T cells (Tregs), B cells, neutrophils, monocytes, macrophages, dendritic cells, NK cells, Mast cells and CD8+ T cells in cancers. Positive correlation in red and negative correlation in blue. * p < 1.05; ** p < 0.01.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) The Spearman correlation heatmap shows the correlation between the expression of NCBP2 and twenty-six immunomodulatory genes in pan-cancer. Red represents positive correlation and blue represents negative correlation. (B–C) Correlations between NCBP2 and tumor mutation burden (TMB) (B) and microsatellite instability (MSI) (C) in pan-cancer. (D) The proportion of patients who receive immunotherapy in the low- and high- NCBP2 subgroup in the IMvigor210 cohort (bladder cancer). (E) Kaplan–Meier curve of low- and high- NCBP2 subgroup in the IMvigor210 cohort. (F) The proportion of patients who receive immunotherapy in the low- and high- NCBP2 subgroup in the GSE91061 cohort (melanoma). (G) Kaplan–Meier curve of low- and high- NCBP2 subgroup in the GSE91061 cohort. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing, Mutagenesis

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Heatmap exhibited the relationship between NCBP2 and stemness indices across cancers. Positive correlation in red and negative correlation in blue. (B) (upper) The relationship between NCBP2 and drug sensitivity calculated by Spearman algorithm. The color of each column represents the p value, whereas the height of each column represents the correlation coefficient. Rs represents the drug sensitivity correlated with the NCBP2 expression. (lower) Dot plot visualized the signal pathways targeted by drugs which were sensitivity (blue) or resistant (red) to the NCBP2. The signal pathways were ranked by the frequency of being targeted. * p < 1.05; ** p < 0.01.

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Expressing

Journal: PeerJ

Article Title: NCBP2 predicts the prognosis and the immunotherapy response of cancers: a pan-cancer analysis

doi: 10.7717/peerj.19050

Figure Lengend Snippet: (A) Correlation analysis between NCBP2 and cancer-related functional states at single cell level according to CancerSEA dataset. (B–D) The association between NCBP2 expression and several biological process in AML (B), UM (C), RB (D). (E–G) t-SNE diagrams were used to visualize NCBP2 expression profiles in AML (E), UM (F), RB (G).

Article Snippet: In order to illustrate the subcellular localization of NCBP2, immunofluorescence staining images of human cancer cell lines (U-251, A-431, U2-OS, MCF7, and PC-3) were utilized (Human Protein Atlas).

Techniques: Functional Assay, Expressing